Pregnancy-related changes in rat cervical glycosaminoglycans.
نویسندگان
چکیده
Non-pregnant and pregnant rats of known gestational age were killed at intervals and their uterine cervices were excised and digested with papain. Glycosaminoglycans thus extracted were separated by cellulose acetate electrophoresis and stained with Alcian Blue. Glycosaminoglycans were identified by comparison with standards and by serial degradation with chondroitin ABC lyase, butyl nitrite and leech hyaluronidase. Dermatan sulphate, hyaluronic acid and heparan sulphate were identified and quantitative determined by densitometry. The overall concentration of glycosaminoglycans changed little during pregnancy. A 3-fold total increase in uronic acid paralleled the increase in cervical weight. Hyaluronate content, however, increased 17-fold, and rose from 6% of total glycosaminoglycans in the non-pregnant state to 33% at term. Furthermore, the ratio of hyaluronate to hydroxyproline increased 10-fold. These changes are consistent with an accumulation of hyaluronate in the interstices between collagen fibres, resulting in the softening of this tissue that is seen in late pregnancy.
منابع مشابه
INFLUENCE OF GLYCOSAMINOGLYCANS ISOLATED FROM THE RAT LIVER WITH VARIOUS STAGES OF CARBON TETRACHLORIDE-INDUCED FIBROSIS UPON COLLAGEN FIBRILLOGENESIS IN VITRO
The influence of glycosaminoglycans (GAG) on collagen fibrillogenesis in vitro was investigated. GAG and collagen were isolated from the liver of rats treated with carbon tetrachloride at various time intervals (3-16 weeks). It was found that GAG obtained from the fibrotic liver accelerated collagen fibril formation. This effect was more evident in a solution of type I collagen than type II...
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متن کاملGlycosaminoglycan Distribution in the Rat Uterine Cervix During the Estrous Cycle
OBJECTIVE To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle. DESIGN Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group): GI - proestrous, GII - estrous, GIII - metaestrous and GIV - diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediat...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 192 1 شماره
صفحات -
تاریخ انتشار 1980